Effects of Ketotifen on an Experimental Model of IgA Nephropathy

Do, Young-Sun; Soon, Eu-Jene; NamGoong, Mee-Kyung

doi:2009.13.2.153

J Korean Soc Pediatr Nephrol > Volume 13(2); 2009 > Article

J Korean Soc Pediatr Nephrol 2009;13(2): 153-160. doi: https://doi.org/10.3339/jkspn.2009.13.2.153

IgA 신증의 실험모델에서 케토티펜의 효과

도영선, 순유진, 남궁미경

¹연세대학교 원주의과대학 소아청소년과학교실
²연세대학교 원주의과대학 소아청소년과학교실
³연세대학교 원주의과대학 소아청소년과학교실

Effects of Ketotifen on an Experimental Model of IgA Nephropathy

Young-Sun Do, Eu-Jene Soon, Mee-Kyung NamGoong

¹Department of Pediatrics and Adolescent Medicine, Wonju Christian Hospital Yonsei University Wonju College of Medicine
²Department of Pediatrics and Adolescent Medicine, Wonju Christian Hospital Yonsei University Wonju College of Medicine
³Department of Pediatrics and Adolescent Medicine, Wonju Christian Hospital Yonsei University Wonju College of Medicine

ABSTRACT

PURPOSE: The intestinal mucosal defect has been known as one of the pathogenicmechanisms of IgA nephropathy. Oral antigens usually induce the activation of Th2 cells and mast cells. These cells secrete cytokines IL-4, IL-5 and TGF-beta, which increase IgA production. Although ketotifen (benzocycloheptathiophene) is an H1 antagonist and a mast cell membrane stabilizer, it could protect the gastrointestinal membrane through inhibiting the production of IL-4, IL-5, PGE2, and LTB4, and decreasing the activity of nitric oxide synthease. Therefore, we have investigated if ketotifen may protect the development of IgA nephropathy with an oral antigen. METHODS: ICR mice were used as an animal model orally with Poliovax only [ketotifen (-)], the other group was given oral ketotifen [ketotifen (+)] in addition to Poliovax. RESULTS: Mesangial IgA deposition developed in 11 out of the 18 mice in the ketotifen (-) group, while in three out of the nine mice in ketotifen (+) group. The mesangial change developed in 16 out of the 18 mice in the ketotifen (-) group, while in five out of the nine mice in the ketotifen (+) group. Serum IL-4 and IL-5 levels were not significantly lower in the latter group than in the former. CONCLUSION: According to the statistical results from the above, ketotifen therapy would be beneficial to reducing mesangial changes in IgA nephropathy.

Key words: Experimental IgA Nephropathy | IL-4 | IL-5 | Benzocycloheptathiophene (Ketotifen)