The Effect of Atorvastatin on the Development of Puromycin Aminonucleoside(PAN)-induced Nephrosis in Rats

Choi, Kwang-Hae; Chung, Hyo-Seuk; Kim, Yong-Jin; Ha, Jeong-Hee; Kim, Heung-Sik; Park, Yong-Hoon

J Korean Soc Pediatr Nephrol > Volume 7(1); 2003 > Article

J Korean Soc Pediatr Nephrol 2003;7(1): 9-15.

Puromycin을 투여한 백서에서 지질 변화가 신증의 진행에 미치는 영향

최광해, 정효석, 김용진, 하정희, 김흥식, 박용훈

¹영남대학교 의과대학 소아과학교실
²영남대학교 의과대학 소아과학교실
³영남대학교 의과대학 병리학교실
⁴영남대학교 의과대학 약리학교실
⁵계명대학교 의과대학 소아과학교실
⁶영남대학교 의과대학 소아과학교실

The Effect of Atorvastatin on the Development of Puromycin Aminonucleoside(PAN)-induced Nephrosis in Rats

Kwang-Hae Choi, Hyo-Seuk Chung, Yong-Jin Kim, Jeong-Hee Ha, Heung-Sik Kim, Yong-Hoon Park

¹Department of Pediatrics, College of Medicine, Yeungnam University
²Department of Pediatrics, College of Medicine, Yeungnam University
³Department of Pathology, College of Medicine, Yeungnam University
⁴Department of Pharmacology, College of Medicine, Yeungnam University
⁵Deportment of Pediatrics, College of Medicine, Keimyung University
⁶Department of Pediatrics, College of Medicine, Yeungnam University

ABSTRACT

PURPOSE: Several studies have suggested that hyperlipidemia might be a causative factor contributing to the progression of initial glomerular injury through the development of glomerulosclerosis. We examined the potential beneficial effect of atorvastatin - which blocks the rate limiting step of cholesterol synthesis by inhibiting HMG-CoA reductase - in PAN- induced nephrosis. MATERIALS AND METHODS: Glomerulosclerosis was induced in Sprague-Dawley male rats by repeated administration of PAN. Sprague-Dawley male rats were divided into 3 groups:group I(control), group II(PAN 20 mg/kg, subcutaneous injection), group III(PAN 20 mg/kg subcutaneous injection and atorvastatin 50 mg/kg/day per oral). On the 11th week, upon sacrifice of the experimental animals, blood sampling, 24-hr urine collection and nephrectomy were performed. RESULTS: Group III had significantly lower BUN and higher serum albumin(30.9+/-17.2 vs. 17.3+/-2.5 mg/dL; 2.3+/-0.1 vs. 2.5+/-0.2 g/dL, P<0.05) compared with group II. In the lipid profiles, group III was associated with a reduction in total cholesterol and LDL(291+/-173 vs. 167+/-72 mg/dL; 57+/-53 vs. 27+/-12 mg/dL, P>0.05) compared with group II. Atorvastatin administration lowered the glomerular sclerosing index significantly(26.2% vs. 13.3%, P<0.05). CONCLUSION: Puromycin-induced glomerulosclerosis could be ameliorated by the reduction of hyperlipidemia with atorvastatin. This suggests that hyperlipidemia contributes to the pathogenesis of glomerulosclerosis.

Key words: Puromycin | Atorvastatin | Glomerulosclerosis | Hyperlipidemia