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Journal of the Korean Society of Pediatric Nephrology 2005;9(2): 119-127.
배양한 사구체 상피세포에서 고농도 당과 후기 당화합물에 의한 P-cadherin의 변화
하태선, 구현회, 이해수, 윤옥자
1충북대학교 의과대학 소아과학교실
2충북대학교 의과대학 소아과학교실
3충북대학교 의과대학 의학연구소
4충북대학교 의과대학 의학연구소
High Glucose and Advanced Glycosylation Endproducts(AGE) Modulate the P-cadherin Expression in Glomerular Epithelial Cells(GEpC)
Tae-Sun Ha, Hyun-Hoe Koo, Hae-Soo Lee, Ok-Ja Yoon
1Department of Pediatrics, Chungbuk National University
2Department of Pediatrics, Chungbuk National University
3Medical Research Institute, Chungbuk National University
4Medical Research Institute, Chungbuk National University
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Purpose : Podocytes are critical in maintaining the filtration barrier of the glomerulus and are dependent on the integrity of slit diaphragm(SD) proteins including nephrin, p-cadherin, and others. Diabetic proteinuric condition demonstrates defects in SD molecules as well as ultrastructural changes in podocytes. We examined the molecular basis for this alteration of SD molecules especially on P-cadherin as a candidate regulating the modulation of pathogenic changes in the barrier to protein filtration.
Methods : To investigate whether high glucose and AGE induce changes in SD, we cultured rat GEpC under normal(5 mM) or high glucose(30 mM) and AGE- or BSA-added conditions and measured the change of P-cadherin expression by Western blotting and RT-PCR.
Results : We found that administration of high glucose decreased the P-cadherin production significantly in the presence or absence of AGE by Western blotting. In RT-PCR high glucose with or without AGE also significantly decreased the expression of P-cadherin mRNA compared to those of controls. Such changes were not seen in the osmotic control.
Conclusion : We suggest that high glucose with or without AGE suppresses the Production of P-cadherin at the transcriptional level and that these changes nay explain the functional changes of SD in diabetic conditions. (J Korean Soc Pediatr Nephrol 2005;9:119-127)
Key words: Diabetic nephropathy | Advanced glycosylation endproducts | p-cadherin | Glomerular epithelial cells | Podocyte
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