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Journal of the Korean Society of Pediatric Nephrology 1999;3(1): 35-41.
Puromycin Aminonucleoside 투여로 초래된 백서신증에 $alpha$-tocopherol이 미치는 영향
서형호, 정태성, 이은실, 신손문, 박용훈, 김용진
1영남대학교 의과대학 소아과학교실
2영남대학교 의과대학 병리학교실
3영남대학교 의과대학 병리학교실
4영남대학교 의과대학 병리학교실
5영남대학교 의과대학 소아과학교실
6영남대학교 의과대학 병리학교실
The Effect of $alpha$-tocopherol in Puromycin Aminonucleoside Induced Nephropathy in Rats
Hyung Ho Seo, Tae Sung Jung, Eun Sil Lee, Son Moon Shin, Yong Hoon Park, Yong Jin Kim
1Department of Pediatrics, Yeungnam University, College of Medicine
2Department of Pathology, Yeungnam University, College of Medicine
3Department of Pathology, Yeungnam University, College of Medicine
4Department of Pathology, Yeungnam University, College of Medicine
5Department of Pediatrics, Yeungnam University, College of Medicine
6Department of Pathology, Yeungnam University, College of Medicine
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Purpose The single administration of PAN(Puromycin-Aminonudeoside) to rats results in nephropathy that are similar to human minimal change nephrotic syndrome. Recently several studies indicate the pathophyslological importance of oxygen free radicals in rats with PAN-induced nephrosis. This study was conducted to evaluate the effect of $alpha$-tocopherol, an oxygen free radical scavenger, on the histologic and biochemical changes of PAN-induced nephrosis in rats.
Methods : Twenty-one Sprague-Dawley rats weighing 180-300 gm were divided into 3 groups. In group I (control group), the rats were given saline intraperitoneally for 12 days, in group II the rats were given PAN 7.5mg/100g of body weight intravenously one time and group III PAN intravenously, followed by $alpha$-tocopherol 0.5 mg/100g of body weight jntramuscularly for 12 days. Twenty four hour urinary protein and creatinine excretion were measured on day 0, 5, 11 and 18. On the 18th day, rats were sacrificed for the determination of total serum protein, albumin and cholesterol levels. To estimate renal injuries by oxygen free radical, lipid peroxide concentration and reduced glutathione were measured in renal cortex. Histological examination in rat glomerular lesions were performed.
Results : From the 5th days of PAN administration, urine protein/creatinine of group II and III were significantly increased compared the group I (P<0.05). But, urine protein/creatinine of group III was significantly lower than group II at 18th days (P<0.05). Total serum protein and albumin of group II were significantly lower than those of group III (P<0.05). Serum cholesterol of group II was significantly higher than that of group III (P<0.05). Lipid peroxide and reduced glutathione in renal cortex of group II were significantly higher than that of group I and III (P<0.05). Electron microscopic strudies of group II showed the loss of epithelial foot processes, but in group III showed preservation of epithelial foot processes.
Conclusion : PAN-induced nephropathy was ameliorated significant recovery of foot process change and reduction of the urinary protein excretion by antioxidant, $alpha$-tocopherol.
Key words: Puromycin aminonucleoside | Antioxidant | Nephropathy
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