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Journal of the Korean Society of Pediatric Nephrology 1998;2(2): 104-109.
사구체신염에서 MIB-1을 이용한 Ki-67 항원의 발현
정우영, 송민섭, 김영주
1인제대학교 의과대학 부산백병원 소아과학교실
2인제대학교 의과대학 부산백병원 소아과학교실
3인제대학교 의과대학 부산백병원 병리학교실
Expression of the Ki-67 antigen Using Monoclonal Antibody MIB-1 in Children with Glomerulonephritis
Woo-Yeong Chung, Min-Seop Song, Young-Ju Kim
1Department of Pediatrics, Pusan Paik Hospital, Inje University, College of Medicine
2Department of Pediatrics, Pusan Paik Hospital, Inje University, College of Medicine
3Department of Pathology, Pusan Paik Hospital, Inje University, College of Medicine
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ABSTRACT
Purpose : The proliferative nuclear antigen Ki-67, present in all cell cycle phases except G0, is a useful marker for the detection of proliferative cells in vivo. MIB I has been found to recognize an antigen in formalin-fixed and wax-embedded material. The aim of this study was to assess the efficacy of MIB-1 expression as a marker of representing the status of mesangial cell proliferation in renal tissues.
Methods : Immunohistochemical staining for Ki-67 Ag using monoclonal antibody MIB-1 (Immunotech,505) were performed in 41 renal tissuses which were obtained by percutaneous renal biopsy done between January 1994 and December 1996.
Results : In both glomeruli and renal tubules, MIB-1 expression was observed only in 2 of 18 ($11.1%$) cases of IgA nephropathy, in 2 of the 4 ($50%$) cases of mebranoproliferative glomerulonephritis, in 4 of the 5 ($80%$) cases of poststreptococcal glomerulonephritis. But MIB-1 expression was not detected in all cases of minimal lesion and membranous nephropathy. Renal tubules In another 7 cases of IgA nephropathy were MIB-1 positive.
Conclusion : MIB-1 expression in renal tissues may relate to the cell proliferation in glomeruli and renal tubules. But the efficacy of MIB-1 expression as a marker of mesangial cell proliferation may reveal a limited value because of it's lower positive rate in IgA nephropathy.
Key words: Ki-67 Antigen | MIB-1 | IgA Nephropathy
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